CCDC138 Knockout cell line (THP-1)

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CCDC138 Knockout cell line (THP-1) Order

CCDC138 Knockout cell line (THP-1)
Catalog#
YKO-HT1075
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CCDC138 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CCDC138 Knockout cell line (THP-1)
Species
Human
Gene Name
CCDC138
Gene ID
165055
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript CCDC138-201)
Validation: PCR+Sanger Sequencing
CCDC138 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CCDC138 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CCDC138 gene knockout CCDC138 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
CCDC138 Knockout cell line (THP-1)
CCDC138 Knockout cell line (THP-1)
Catalog#
YKO-HT1075
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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